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rabbit anti hdc antibody  (OriGene)


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    OriGene rabbit anti hdc antibody
    Rabbit Anti Hdc Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+hdc/Histidine+decarboxylase+(HDC)+Rabbit+Polyclonal+Antibody/pmc11310318-412-15-21
    Average 92 stars, based on 4 article reviews
    rabbit anti hdc antibody - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: Sensitivity of hypocretin system to chronic alcohol exposure: A human and animal study
    Article Snippet: .. After thorough washing with PBS, the sections were placed for 2 h in either 1.5% normal goat serum (NGS for Hcrt, MCH and HDC) or 1% rabbit serum (NRS for Iba-1) in PBS containing 0.25% Triton X (PBST) and incubated for 72 h at 4°C with either rabbit anti-Hcrt-1 (H-003-30, Phoenix Pharmaceuticals, USA, Lot# 01651-11, 1:10000), rabbit anti-MCH (Cat No. H -070-47, Phoenix Pharmaceuticals, USA, Lot# 01629-5, 1:20000), rabbit anti-HDC (Cat No. EUD2601, OriGene Technologies, Lot#17106, 1:5000) or goat anti-lba-1 antibody (ab5076, Abcam, USA, Lot # GR3322398-1, 1:5000) PBST, 1% NGS or 1% NRS. .. Sections were then incubated for 120 min in a secondary antibody (biotinylated goat anti-rabbit IgG or rabbit anti-goat IgG, 1:400, Vector Laboratories, Burlingame, CA) in PBST, avidin–biotin complex (120 min, ABC Elite Kit, 1:300, Vector Laboratories, in PBS) and developed with the diaminobenzidine tetrahydrochloride (DAB) method, which consisted of tissue immersion in 0.02% DAB and 0.03% hydrogen peroxide for 8 min. A common practice in histology laboratories that use the DAB method to develop immunohistochemical procedures, is to periodically examine the tissue sections “under the microscope” during the developing process and remove the sections from the DAB solution once neurons are visualized.

    Incubation:

    Article Title: Sensitivity of hypocretin system to chronic alcohol exposure: A human and animal study
    Article Snippet: .. After thorough washing with PBS, the sections were placed for 2 h in either 1.5% normal goat serum (NGS for Hcrt, MCH and HDC) or 1% rabbit serum (NRS for Iba-1) in PBS containing 0.25% Triton X (PBST) and incubated for 72 h at 4°C with either rabbit anti-Hcrt-1 (H-003-30, Phoenix Pharmaceuticals, USA, Lot# 01651-11, 1:10000), rabbit anti-MCH (Cat No. H -070-47, Phoenix Pharmaceuticals, USA, Lot# 01629-5, 1:20000), rabbit anti-HDC (Cat No. EUD2601, OriGene Technologies, Lot#17106, 1:5000) or goat anti-lba-1 antibody (ab5076, Abcam, USA, Lot # GR3322398-1, 1:5000) PBST, 1% NGS or 1% NRS. .. Sections were then incubated for 120 min in a secondary antibody (biotinylated goat anti-rabbit IgG or rabbit anti-goat IgG, 1:400, Vector Laboratories, Burlingame, CA) in PBST, avidin–biotin complex (120 min, ABC Elite Kit, 1:300, Vector Laboratories, in PBS) and developed with the diaminobenzidine tetrahydrochloride (DAB) method, which consisted of tissue immersion in 0.02% DAB and 0.03% hydrogen peroxide for 8 min. A common practice in histology laboratories that use the DAB method to develop immunohistochemical procedures, is to periodically examine the tissue sections “under the microscope” during the developing process and remove the sections from the DAB solution once neurons are visualized.



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    ( A ) Top: Schematic of hM3Dq-mCherry microinjection into the TMN of HDC-Cre mice. Bottom: Neuropixels recordings in the optic tract before and after CNO. Bottom: <t>Immunostaining</t> showing hM3Dq-mCherry + (red) and HDC + (green) neurons. ( B ) Left: Tracing of the probe of a recorded mouse. Right: High magnification. ( C ) Polar plots of an example DSGC’s axon in a control mouse to moving gratings at different temporal frequencies, before (left) and after (right) CNO. ( D ) Mean firing rates of the cell in (C) to different temporal frequencies denoted on the left, before (left) and after (right) CNO. Notions as in . ( E ) Mean spike waveform (black) with 100 individual spikes (gray) of the cell in (C). ( F ) Receptive field calculated in response to white noise stimulation of the cell in (C). ( G ) Population data (mean ± SEM) of the first harmonic magnitude of the FFT at the stimulus frequency as a function of temporal frequency for DSGCs axons in control mice ( n = 31; the effect of CNO is not significant for the two-way repeated-measures ANOVA). ( H to K ) Same as in (C) to (F) except for an hM3Dq mouse. ( L ) Same as (G) except for hM3Dq mice ( n = 59, P = 0.001 for 4 Hz). Two-way repeated-measures ANOVA with Bonferroni post hoc correction for multiple comparisons. ( M ) Schematic of the setup for OMR. ( N ) OMR index calculated in response to stimuli moving at 6°, 12°, and 35°/s in C57BL/6J mice ( n = 16) treated with water (gray) or H 1 R antagonist (green). Two-way repeated-measures ANOVA with Tukey’s post hoc correction for multiple comparisons. P = 0.0467 and P = 0.0079 for H 1 R antagonist versus Pre and Post. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: OPT, optic tract; D, R, U, and L, down, right, up, and left, respectively; PD, preferred direction; ND, null direction. (A) and (M) adapted from BioRender.com .
    Rabbit Polyclonal Anti Hdc, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti hdc antibody  (OriGene)
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    ( A ) Top: Schematic of hM3Dq-mCherry microinjection into the TMN of HDC-Cre mice. Bottom: Neuropixels recordings in the optic tract before and after CNO. Bottom: <t>Immunostaining</t> showing hM3Dq-mCherry + (red) and HDC + (green) neurons. ( B ) Left: Tracing of the probe of a recorded mouse. Right: High magnification. ( C ) Polar plots of an example DSGC’s axon in a control mouse to moving gratings at different temporal frequencies, before (left) and after (right) CNO. ( D ) Mean firing rates of the cell in (C) to different temporal frequencies denoted on the left, before (left) and after (right) CNO. Notions as in . ( E ) Mean spike waveform (black) with 100 individual spikes (gray) of the cell in (C). ( F ) Receptive field calculated in response to white noise stimulation of the cell in (C). ( G ) Population data (mean ± SEM) of the first harmonic magnitude of the FFT at the stimulus frequency as a function of temporal frequency for DSGCs axons in control mice ( n = 31; the effect of CNO is not significant for the two-way repeated-measures ANOVA). ( H to K ) Same as in (C) to (F) except for an hM3Dq mouse. ( L ) Same as (G) except for hM3Dq mice ( n = 59, P = 0.001 for 4 Hz). Two-way repeated-measures ANOVA with Bonferroni post hoc correction for multiple comparisons. ( M ) Schematic of the setup for OMR. ( N ) OMR index calculated in response to stimuli moving at 6°, 12°, and 35°/s in C57BL/6J mice ( n = 16) treated with water (gray) or H 1 R antagonist (green). Two-way repeated-measures ANOVA with Tukey’s post hoc correction for multiple comparisons. P = 0.0467 and P = 0.0079 for H 1 R antagonist versus Pre and Post. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: OPT, optic tract; D, R, U, and L, down, right, up, and left, respectively; PD, preferred direction; ND, null direction. (A) and (M) adapted from BioRender.com .
    Rabbit Anti Hdc Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+hdc/Histidine+decarboxylase+(HDC)+Rabbit+Polyclonal+Antibody/pmc11310318-412-15-21
    Average 92 stars, based on 1 article reviews
    rabbit anti hdc antibody - by Bioz Stars, 2026-09
    92/100 stars
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    rabbit anti-hdc  (American Research Products)
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    ( A ) Top: Schematic of hM3Dq-mCherry microinjection into the TMN of HDC-Cre mice. Bottom: Neuropixels recordings in the optic tract before and after CNO. Bottom: <t>Immunostaining</t> showing hM3Dq-mCherry + (red) and HDC + (green) neurons. ( B ) Left: Tracing of the probe of a recorded mouse. Right: High magnification. ( C ) Polar plots of an example DSGC’s axon in a control mouse to moving gratings at different temporal frequencies, before (left) and after (right) CNO. ( D ) Mean firing rates of the cell in (C) to different temporal frequencies denoted on the left, before (left) and after (right) CNO. Notions as in . ( E ) Mean spike waveform (black) with 100 individual spikes (gray) of the cell in (C). ( F ) Receptive field calculated in response to white noise stimulation of the cell in (C). ( G ) Population data (mean ± SEM) of the first harmonic magnitude of the FFT at the stimulus frequency as a function of temporal frequency for DSGCs axons in control mice ( n = 31; the effect of CNO is not significant for the two-way repeated-measures ANOVA). ( H to K ) Same as in (C) to (F) except for an hM3Dq mouse. ( L ) Same as (G) except for hM3Dq mice ( n = 59, P = 0.001 for 4 Hz). Two-way repeated-measures ANOVA with Bonferroni post hoc correction for multiple comparisons. ( M ) Schematic of the setup for OMR. ( N ) OMR index calculated in response to stimuli moving at 6°, 12°, and 35°/s in C57BL/6J mice ( n = 16) treated with water (gray) or H 1 R antagonist (green). Two-way repeated-measures ANOVA with Tukey’s post hoc correction for multiple comparisons. P = 0.0467 and P = 0.0079 for H 1 R antagonist versus Pre and Post. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: OPT, optic tract; D, R, U, and L, down, right, up, and left, respectively; PD, preferred direction; ND, null direction. (A) and (M) adapted from BioRender.com .
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    rabbit anti hdc  (OriGene)
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    ( A ) Top: Schematic of hM3Dq-mCherry microinjection into the TMN of HDC-Cre mice. Bottom: Neuropixels recordings in the optic tract before and after CNO. Bottom: <t>Immunostaining</t> showing hM3Dq-mCherry + (red) and HDC + (green) neurons. ( B ) Left: Tracing of the probe of a recorded mouse. Right: High magnification. ( C ) Polar plots of an example DSGC’s axon in a control mouse to moving gratings at different temporal frequencies, before (left) and after (right) CNO. ( D ) Mean firing rates of the cell in (C) to different temporal frequencies denoted on the left, before (left) and after (right) CNO. Notions as in . ( E ) Mean spike waveform (black) with 100 individual spikes (gray) of the cell in (C). ( F ) Receptive field calculated in response to white noise stimulation of the cell in (C). ( G ) Population data (mean ± SEM) of the first harmonic magnitude of the FFT at the stimulus frequency as a function of temporal frequency for DSGCs axons in control mice ( n = 31; the effect of CNO is not significant for the two-way repeated-measures ANOVA). ( H to K ) Same as in (C) to (F) except for an hM3Dq mouse. ( L ) Same as (G) except for hM3Dq mice ( n = 59, P = 0.001 for 4 Hz). Two-way repeated-measures ANOVA with Bonferroni post hoc correction for multiple comparisons. ( M ) Schematic of the setup for OMR. ( N ) OMR index calculated in response to stimuli moving at 6°, 12°, and 35°/s in C57BL/6J mice ( n = 16) treated with water (gray) or H 1 R antagonist (green). Two-way repeated-measures ANOVA with Tukey’s post hoc correction for multiple comparisons. P = 0.0467 and P = 0.0079 for H 1 R antagonist versus Pre and Post. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: OPT, optic tract; D, R, U, and L, down, right, up, and left, respectively; PD, preferred direction; ND, null direction. (A) and (M) adapted from BioRender.com .
    Rabbit Anti Hdc, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
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    rabbit anti-hdc eud2601  (OriGene)
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    OriGene rabbit anti-hdc eud2601
    ( A ) Top: Schematic of hM3Dq-mCherry microinjection into the TMN of HDC-Cre mice. Bottom: Neuropixels recordings in the optic tract before and after CNO. Bottom: <t>Immunostaining</t> showing hM3Dq-mCherry + (red) and HDC + (green) neurons. ( B ) Left: Tracing of the probe of a recorded mouse. Right: High magnification. ( C ) Polar plots of an example DSGC’s axon in a control mouse to moving gratings at different temporal frequencies, before (left) and after (right) CNO. ( D ) Mean firing rates of the cell in (C) to different temporal frequencies denoted on the left, before (left) and after (right) CNO. Notions as in . ( E ) Mean spike waveform (black) with 100 individual spikes (gray) of the cell in (C). ( F ) Receptive field calculated in response to white noise stimulation of the cell in (C). ( G ) Population data (mean ± SEM) of the first harmonic magnitude of the FFT at the stimulus frequency as a function of temporal frequency for DSGCs axons in control mice ( n = 31; the effect of CNO is not significant for the two-way repeated-measures ANOVA). ( H to K ) Same as in (C) to (F) except for an hM3Dq mouse. ( L ) Same as (G) except for hM3Dq mice ( n = 59, P = 0.001 for 4 Hz). Two-way repeated-measures ANOVA with Bonferroni post hoc correction for multiple comparisons. ( M ) Schematic of the setup for OMR. ( N ) OMR index calculated in response to stimuli moving at 6°, 12°, and 35°/s in C57BL/6J mice ( n = 16) treated with water (gray) or H 1 R antagonist (green). Two-way repeated-measures ANOVA with Tukey’s post hoc correction for multiple comparisons. P = 0.0467 and P = 0.0079 for H 1 R antagonist versus Pre and Post. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: OPT, optic tract; D, R, U, and L, down, right, up, and left, respectively; PD, preferred direction; ND, null direction. (A) and (M) adapted from BioRender.com .
    Rabbit Anti Hdc Eud2601, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Top: Schematic of hM3Dq-mCherry microinjection into the TMN of HDC-Cre mice. Bottom: Neuropixels recordings in the optic tract before and after CNO. Bottom: Immunostaining showing hM3Dq-mCherry + (red) and HDC + (green) neurons. ( B ) Left: Tracing of the probe of a recorded mouse. Right: High magnification. ( C ) Polar plots of an example DSGC’s axon in a control mouse to moving gratings at different temporal frequencies, before (left) and after (right) CNO. ( D ) Mean firing rates of the cell in (C) to different temporal frequencies denoted on the left, before (left) and after (right) CNO. Notions as in . ( E ) Mean spike waveform (black) with 100 individual spikes (gray) of the cell in (C). ( F ) Receptive field calculated in response to white noise stimulation of the cell in (C). ( G ) Population data (mean ± SEM) of the first harmonic magnitude of the FFT at the stimulus frequency as a function of temporal frequency for DSGCs axons in control mice ( n = 31; the effect of CNO is not significant for the two-way repeated-measures ANOVA). ( H to K ) Same as in (C) to (F) except for an hM3Dq mouse. ( L ) Same as (G) except for hM3Dq mice ( n = 59, P = 0.001 for 4 Hz). Two-way repeated-measures ANOVA with Bonferroni post hoc correction for multiple comparisons. ( M ) Schematic of the setup for OMR. ( N ) OMR index calculated in response to stimuli moving at 6°, 12°, and 35°/s in C57BL/6J mice ( n = 16) treated with water (gray) or H 1 R antagonist (green). Two-way repeated-measures ANOVA with Tukey’s post hoc correction for multiple comparisons. P = 0.0467 and P = 0.0079 for H 1 R antagonist versus Pre and Post. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: OPT, optic tract; D, R, U, and L, down, right, up, and left, respectively; PD, preferred direction; ND, null direction. (A) and (M) adapted from BioRender.com .

    Journal: Science Advances

    Article Title: Top-down modulation of the retinal code via histaminergic neurons of the hypothalamus

    doi: 10.1126/sciadv.adk4062

    Figure Lengend Snippet: ( A ) Top: Schematic of hM3Dq-mCherry microinjection into the TMN of HDC-Cre mice. Bottom: Neuropixels recordings in the optic tract before and after CNO. Bottom: Immunostaining showing hM3Dq-mCherry + (red) and HDC + (green) neurons. ( B ) Left: Tracing of the probe of a recorded mouse. Right: High magnification. ( C ) Polar plots of an example DSGC’s axon in a control mouse to moving gratings at different temporal frequencies, before (left) and after (right) CNO. ( D ) Mean firing rates of the cell in (C) to different temporal frequencies denoted on the left, before (left) and after (right) CNO. Notions as in . ( E ) Mean spike waveform (black) with 100 individual spikes (gray) of the cell in (C). ( F ) Receptive field calculated in response to white noise stimulation of the cell in (C). ( G ) Population data (mean ± SEM) of the first harmonic magnitude of the FFT at the stimulus frequency as a function of temporal frequency for DSGCs axons in control mice ( n = 31; the effect of CNO is not significant for the two-way repeated-measures ANOVA). ( H to K ) Same as in (C) to (F) except for an hM3Dq mouse. ( L ) Same as (G) except for hM3Dq mice ( n = 59, P = 0.001 for 4 Hz). Two-way repeated-measures ANOVA with Bonferroni post hoc correction for multiple comparisons. ( M ) Schematic of the setup for OMR. ( N ) OMR index calculated in response to stimuli moving at 6°, 12°, and 35°/s in C57BL/6J mice ( n = 16) treated with water (gray) or H 1 R antagonist (green). Two-way repeated-measures ANOVA with Tukey’s post hoc correction for multiple comparisons. P = 0.0467 and P = 0.0079 for H 1 R antagonist versus Pre and Post. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: OPT, optic tract; D, R, U, and L, down, right, up, and left, respectively; PD, preferred direction; ND, null direction. (A) and (M) adapted from BioRender.com .

    Article Snippet: Immunohistochemical analysis of virus expression (reporter gene, mCherry or tdTomato) in combination with the identification of histaminergic neurons via HDC immunohistochemistry was performed on mouse brain slices 3 to 4 weeks after the injection (primary antibody: rabbit polyclonal anti-HDC, 1:300, PROGEN Biotechnik GmbH, cat. no. 16045; secondary antibody: donkey anti-rabbit 488, 1:200, Invitrogen A21206).

    Techniques: Microinjection, Immunostaining, Control